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  • dawn1313
    Member
    • Aug 2015
    • 21

    #1

    How to get Annotation file of virus for Sashimi plot

    Hi,

    I am trying to do Sashimi plot from miso but my virus annotation file (see below) doesn't fit its annotation format. Can you help to figure it out? Thank you!
    ------------------------
    $ head ref_annotation1.gff3
    ##gff-version 3
    # sequence-region gi|157779983|gb|EF999921.1| 1 229050
    # conversion-by bp_genbank2gff3.pl
    # organism Human herpesvirus 5
    # date 18-JAN-2008
    # Note Human herpesvirus 5 strain TB40/E clone TB40-BAC4, complete sequence.
    gi|157779983|gb|EF999921.1| GenBank region 1 229050 . + . ID=gi|157779983|gb|EF999921.1|;Name=gi|157779983|gb|EF999921.1|;Dbxref=taxon:10359;Note=Human herpesvirus 5 strain TB40/E clone TB40-BAC4%2C complete sequence.,passage 22 in human endothelial cells;clone=TB40-BAC4;country=Germany;date=18-JAN-2008;mol_type=genomic DNA;organism=Human herpesvirus 5;strain=TB40/E
    gi|157779983|gb|EF999921.1| GenBank gene 331 1008 . - 0 ID=US7;Name=US7;Dbxref=GI:157779984;codon_start=1;product=US7;protein_id=ABV71498.1;translation=length.225
    gi|157779983|gb|EF999921.1| GenBank gene 1210 1890 . - 0 ID=US8;Name=US8;Dbxref=GI:157779985;codon_start=1;product=US8;protein_id=ABV71499.1;translation=length.226

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