Hi all,
I made a bigWig file from a published ChIP-Seq data using Homer software. And, I uploaded the "trackfileoutput.txt" to UCSC genome browser (on "Paste URLs or data" in Add Custom Tracks). But, I got an error message such as "Error https://dl.dropboxusercontent.com/u/...ucsc.bigWig.bw is not a big wig file").
The "trackfileoutput.txt" contains the header information that is uploaded as a custom track to UCSC at below;
track type=bigWig name="tag_forebrain Total Tags = 5.96e+06, normalized to 1.00e+07" description="tag_forebrain Total Tags = 5.96e+06, normalized to 1.00e+07" bigDataUrl=https://dl.dropboxusercontent.com/u/167314749/tag_forebrain.ucsc.bigWig.bw color=15,94,94 visibility=full yLineOnOff=on autoScale=on yLineMark="0.0" alwaysZero=on graphType=bar maxHeightPixels=128:75:11 windowingFunction=maximum smoothingWindow=off
And as an example, the format of the "tag_forebrain.ucsc.bigWig.bw" is at blow;
chr1 3000020 3000136 1.45
chr1 3000197 3000313 2.89
chr1 3000940 3001056 1.45
chr1 3001085 3001201 2.89
chr1 3001741 3001857 1.45
chr1 3005707 3005823 1.45
chr1 3008543 3008659 1.45
chr1 3010571 3010687 1.45
chr1 3013107 3013190 1.45
chr1 3013190 3013223 2.89
chr1 3013223 3013233 1.45
Does anyone know about this type of issue? Please share your information to resolve my problem. Thank you.
taehee
I made a bigWig file from a published ChIP-Seq data using Homer software. And, I uploaded the "trackfileoutput.txt" to UCSC genome browser (on "Paste URLs or data" in Add Custom Tracks). But, I got an error message such as "Error https://dl.dropboxusercontent.com/u/...ucsc.bigWig.bw is not a big wig file").
The "trackfileoutput.txt" contains the header information that is uploaded as a custom track to UCSC at below;
track type=bigWig name="tag_forebrain Total Tags = 5.96e+06, normalized to 1.00e+07" description="tag_forebrain Total Tags = 5.96e+06, normalized to 1.00e+07" bigDataUrl=https://dl.dropboxusercontent.com/u/167314749/tag_forebrain.ucsc.bigWig.bw color=15,94,94 visibility=full yLineOnOff=on autoScale=on yLineMark="0.0" alwaysZero=on graphType=bar maxHeightPixels=128:75:11 windowingFunction=maximum smoothingWindow=off
And as an example, the format of the "tag_forebrain.ucsc.bigWig.bw" is at blow;
chr1 3000020 3000136 1.45
chr1 3000197 3000313 2.89
chr1 3000940 3001056 1.45
chr1 3001085 3001201 2.89
chr1 3001741 3001857 1.45
chr1 3005707 3005823 1.45
chr1 3008543 3008659 1.45
chr1 3010571 3010687 1.45
chr1 3013107 3013190 1.45
chr1 3013190 3013223 2.89
chr1 3013223 3013233 1.45
Does anyone know about this type of issue? Please share your information to resolve my problem. Thank you.
taehee
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