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  • Upload Illumina raw seqs to NCBI SRA

    Hi, I am switching from 454 sequencing to Illumina. Now, I am going to upload my Illumina amplicon seqs to NCBI SRA.

    What format should I use? For 454, I normally upload raw SFF file. Also, I split my total SFF file into each sample and link each sample to its ID on NCBI.

    However, for illumina, I have a total file in FASTQ format. If I slit it into individual file, it will give me individual FASTA file (after de-multiplexier).

    Should I upload individual FASTA file? Or total FASTQ file?

    What format do you normally use for Illumina sequencing?

    Thanks

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