The difference between sequence and quality might not matter for the error
Hi All,
I got the same error "Reads file contained a pattern with more than 1024 quality values." with Bowtie 0.12.3
My data have 76bp / the same length in quality:
ILLUMINA-1A5BF1 1 8 61 12450 2086 0 1 TGCTGCGCTGTGATTTCTCGCTGGCAGACTTGGGTTGGCTTTGCTGAGGGGACGTGAGACATTGTATCAGGGGCCA bbbbbbbbbbbbbbbbbbbbbbbbbbbcbcbbbbbbbbbbbbbbbbbbb`bbbIbbbb_bbbbabbb]bbbbbbbb 1
After I convert them to fastq format (76/76) like this:
@ILLUMINA-1A5BF1:8:1:1303:18887#0/1
TAGGAGGGTGACCTGAAGAGTGGAAGGAAGAGTCAGGAATACTCAGAAGAACCTGTGCATATAGGCCAGGCCCGAC
+ILLUMINA-1A5BF1:8:1:1303:18887#0/1
aaaa_aaaaaaaaaa]aaYaaaaaaaa_`a_a_a_aaXaa_a`[_aa_`N`aa_`]a]`aXHVV]a`^X]YQHYVa
I got the error.
I guess that the count difference between sequence and quality might not matter for the error.
It'd be greatly appreciated if someone can help me.
Cheers,
KJ
Originally posted by Ben Langmead
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I got the same error "Reads file contained a pattern with more than 1024 quality values." with Bowtie 0.12.3
My data have 76bp / the same length in quality:
ILLUMINA-1A5BF1 1 8 61 12450 2086 0 1 TGCTGCGCTGTGATTTCTCGCTGGCAGACTTGGGTTGGCTTTGCTGAGGGGACGTGAGACATTGTATCAGGGGCCA bbbbbbbbbbbbbbbbbbbbbbbbbbbcbcbbbbbbbbbbbbbbbbbbb`bbbIbbbb_bbbbabbb]bbbbbbbb 1
After I convert them to fastq format (76/76) like this:
@ILLUMINA-1A5BF1:8:1:1303:18887#0/1
TAGGAGGGTGACCTGAAGAGTGGAAGGAAGAGTCAGGAATACTCAGAAGAACCTGTGCATATAGGCCAGGCCCGAC
+ILLUMINA-1A5BF1:8:1:1303:18887#0/1
aaaa_aaaaaaaaaa]aaYaaaaaaaa_`a_a_a_aaXaa_a`[_aa_`N`aa_`]a]`aXHVV]a`^X]YQHYVa
I got the error.
I guess that the count difference between sequence and quality might not matter for the error.
It'd be greatly appreciated if someone can help me.
Cheers,
KJ
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