Hi everybody!
I starting using bowtie today, i wanted to align csfasta + qual file width the bowtie.
I build the reference fasta file width the bowtie-build, after that i try to align the csfasta+qual file to the reference file(s), but i have error massege.
The bowtie-build command:
The bowtie command:
And the error command with my bowtie commnad:
The csfasta file contains only short reads, every sequances are 50 bp long.
My question is that what is the error mean? I try to search this error message but don't found anything.
I installed the bowtie width the following way:
I really appreciate any help/answer.
Thank you!
I starting using bowtie today, i wanted to align csfasta + qual file width the bowtie.
I build the reference fasta file width the bowtie-build, after that i try to align the csfasta+qual file to the reference file(s), but i have error massege.
The bowtie-build command:
bowtie-build -C reference_genom.fa ref/reference_genom
bowtie -C ref/reference_genom -f read.csfasta -Q quality.qual -S align.sam
bowtie -C ref/reference_genom -f read.csfasta -Q quality.qual -S align.sam
/usr/include/seqan/sequence/string_base.h:237 Assertion failed : static_cast<TStringPos>(pos) < static_cast<TStringPos>(length(me)) was: 48 >= 48 (Trying to access an element behind the last one!)
Aborted
/usr/include/seqan/sequence/string_base.h:237 Assertion failed : static_cast<TStringPos>(pos) < static_cast<TStringPos>(length(me)) was: 48 >= 48 (Trying to access an element behind the last one!)
Aborted
My question is that what is the error mean? I try to search this error message but don't found anything.
I installed the bowtie width the following way:
sudo apt-get install bowtie
Thank you!
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