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  • TPH
    Member
    • Jan 2016
    • 19

    cummeRbund error

    Hello,

    After completing all the analysis steps from Tophat to Cudiff, I am trying to plot the data using CummeRbund. But I get this error message saying "semi-transparency is not supported on this device: reported only once per page".
    I work in a cluster using Linux and use R/3.2.2 compiled with Cairo. Could you please help me to fugure out the issue here.

    These are the commands and their outputs I received.

    $ module load R/3.2.2
    $ R

    >library(cummeRbund)
    >cuff_data <‐ readCufflinks('/Path to cuffdiff output folder/cuffdiff_out')
    >csDensity(genes(cuff_data))

    Removed 8804 rows containing non-finite values (stat_density).
    2: In grid.Call.graphics(L_polygon, x$x, x$y, index) :
    semi-transparency is not supported on this device: reported only once per page




    ---------------------------------------------------------------

    I feel like there is an issue with the cummeRbund loading. But I can't figure out what is it. This is the output I get when cummeRbund Loads.



    Loading required package: BiocGenerics
    Loading required package: parallel

    Attaching package: ‘BiocGenerics’

    The following objects are masked from ‘packagearallel’:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

    The following objects are masked from ‘package:stats’:

    IQR, mad, xtabs

    The following objects are masked from ‘package:base’:

    anyDuplicated, append, as.data.frame, as.vector, cbind, colnames,
    do.call, duplicated, eval, evalq, Filter, Find, get, grep, grepl,
    intersect, is.unsorted, lapply, lengths, Map, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
    rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unlist, unsplit

    Loading required package: RSQLite
    Loading required package: DBI
    Loading required package: ggplot2
    Loading required package: reshape2
    Loading required package: fastcluster

    Attaching package: ‘fastcluster’

    The following object is masked from ‘package:stats’:

    hclust

    Loading required package: rtracklayer
    Loading required package: GenomicRanges
    Loading required package: S4Vectors
    Loading required package: stats4
    Loading required package: IRanges
    Loading required package: GenomeInfoDb
    Loading required package: Gviz
    Loading required package: grid

    Attaching package: ‘cummeRbund’

    The following object is masked from ‘package:GenomicRanges’:

    promoters

    The following object is masked from ‘package:IRanges’:

    promoters

    The following object is masked from ‘package:BiocGenerics’:

    Conditions



    Could you please help.

    Thank you very much.

    TPH
    Last edited by TPH; 01-21-2016, 12:37 PM.

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