Hi All,
I downloaded a few public RNA-seq data, and mapped them to the genome and viewed in IGV . As I don't know if the libraries were constructed strand-specifically. Is it possible to judge whether the data are strand-specific or not from the alignment (see an example figure below)? I feel it is (but would like to confirm). Because almost all the genes are of reads of the same direction.
Thanks,
Rui
I downloaded a few public RNA-seq data, and mapped them to the genome and viewed in IGV . As I don't know if the libraries were constructed strand-specifically. Is it possible to judge whether the data are strand-specific or not from the alignment (see an example figure below)? I feel it is (but would like to confirm). Because almost all the genes are of reads of the same direction.
Thanks,
Rui
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