Hi,
I am using HTSeq for counting of my paired-end bam files. Some reads in the file do not have mate reads because they have been discarded by trimmomatic for some reasons.
Can HTSeq somehow handle this as I am getting Errors saying "Sequence of paired-end alignments expected, but got single-end alignment."?
Best, Christian
I am using HTSeq for counting of my paired-end bam files. Some reads in the file do not have mate reads because they have been discarded by trimmomatic for some reasons.
Can HTSeq somehow handle this as I am getting Errors saying "Sequence of paired-end alignments expected, but got single-end alignment."?
Best, Christian
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