Originally posted by NextGenSeq
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Originally posted by NextGenSeq View PostCLC Bio is a bit buggy. You have to force import your reference genome as normal sequence data NOT next gen sequence data. Once you do that it is easy to annotate it using a GFF file. I tried attach the pdf describing it but this website gave an error message. If you private message me with your email address I can email it to you.
Hi guys,
I'm having the same problem using CLC ... and I couldn't find any viable solution...About this pdf... is it from Clc? Because not even using their example data from the 'Annotation Plug-in' we could make it work.
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If you're looking to do basic analysis have a look at some open solutions e.g. DIYA. It's quite simple to run a set of programs in serial. The hard part with annotation is manual curation and Artemis is a good tried and tested option. Geneious has some good offerings and it is prettier.
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