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I am using bowtie and have been outputting .bwt files but now need to convert those files to .sam format. I know bowtie has a .sam output option (-S) which I am now using, however I need to convert all the files I have produced so far using the .bwt format into SAM files so I don't lose all the work I have done so far. Using bowtie2sam.pl I can easily convert the files but the new files don't have headers, which is I need for the SAM -> BAM conversion. Is there a way to convert the files that creates headers or would it be better to manually add new headers with a loop of some kind? Thanks for the help!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
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