Hi~ all ^^
I'm currently trying to assemble genome sequence and I have about 300 million Solexa reads (Paired-end; 220bp insert size).
When I using Velvet for assembling, I've got an error as below:
---------------------------------------------------------------------------------
velvetg: Can't calloc 18446744072010747658 InsertionMarkers totalling
18446744046528688288 bytes: Cannot allocate memory
Reading roadmap file ./Roadmaps
301083362 roadmaps reads
Creating insertion markers
---------------------------------------------------------------------------------
So I try to use other assemblers. If somebody tried to assemble paired-end Solexa reads and successfully completed, please tell me about the assembler and run command.
Server specification: 12 CPU, 72GB RAM
Thanks for any comments.
I'm currently trying to assemble genome sequence and I have about 300 million Solexa reads (Paired-end; 220bp insert size).
When I using Velvet for assembling, I've got an error as below:
---------------------------------------------------------------------------------
velvetg: Can't calloc 18446744072010747658 InsertionMarkers totalling
18446744046528688288 bytes: Cannot allocate memory
Reading roadmap file ./Roadmaps
301083362 roadmaps reads
Creating insertion markers
---------------------------------------------------------------------------------
So I try to use other assemblers. If somebody tried to assemble paired-end Solexa reads and successfully completed, please tell me about the assembler and run command.
Server specification: 12 CPU, 72GB RAM
Thanks for any comments.
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