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htseq-count ERROR in GFF File?
Hello guys,
I'm trying to do a summary of counts in usegalaxy.org. I convert Fatq groomer to sam with Map with BWA for Illumina (Galaxy Version 1.2.3) with a camponotus floridanus genome and
now i have a sam file:
~98,000,000 lines, 24,027 comments
format: sam database?
BWA Version: 0.5.9-r16 BWA run on single-end data
And a gff3 file from http://hymenopteragenome.org/campono...tium_datasets:
~190,000 lines
format: gff3 database?
uploaded gff3 file
When i do a summary of counts with htseq-count
:
Fatal error: Unknown error occured
Error occured when processing GFF file (line 5 of file /galaxy-repl/main/files/016/000/dataset_16000531.dat):
Feature CFLO10001-RA does not contain a 'gene_id' attribute
[Exception type: ValueError, raised in count.
Thanks you everyone!
I'm trying to do a summary of counts in usegalaxy.org. I convert Fatq groomer to sam with Map with BWA for Illumina (Galaxy Version 1.2.3) with a camponotus floridanus genome and
now i have a sam file:
~98,000,000 lines, 24,027 comments
format: sam database?
BWA Version: 0.5.9-r16 BWA run on single-end data
And a gff3 file from http://hymenopteragenome.org/campono...tium_datasets:
~190,000 lines
format: gff3 database?
uploaded gff3 file
When i do a summary of counts with htseq-count
:Fatal error: Unknown error occured
Error occured when processing GFF file (line 5 of file /galaxy-repl/main/files/016/000/dataset_16000531.dat):
Feature CFLO10001-RA does not contain a 'gene_id' attribute
[Exception type: ValueError, raised in count.
Thanks you everyone!