Hi all,
I have received two paired end fastq files to analyze and when I look at the header I realized the following:
Illumina explains the header here:
and as I have always seen the third from last character tells you about the read number; 1 for single read or read 2 of paired-end. In this case I have a number 3 as you can see.
Has anyone seen this? Maybe it is a very common issue but I never saw it and I am suspicious about it.
Thanks in advance,
I have received two paired end fastq files to analyze and when I look at the header I realized the following:
Code:
File 1 @HWI-ST1131:130302:D1RGUACXX:6:1101:1133:2071 1:N:0: ... File 2 @HWI-ST1131:130302:D1RGUACXX:6:1101:1133:2071 3:N:0: ...
and as I have always seen the third from last character tells you about the read number; 1 for single read or read 2 of paired-end. In this case I have a number 3 as you can see.
Has anyone seen this? Maybe it is a very common issue but I never saw it and I am suspicious about it.
Thanks in advance,
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