-
The wiggle score can be anything, it's a track type, not a type of measurement. For a NGS dataset, it can be raw reads (i.e. how many reads cover that bp), or normalized RPM, with or without multireads, with multiread contribution to the score estimated in some convoluted way based on unique read abundance in the neighborhood, etc, etc. It all depends on how it was generated. -
In the .wig files we generate it's a count of the reads that either start at or cover that location. I suppose it could also be a normalized read count.Leave a comment:
-
wiggle score
Hi,
Does anyone know how the scores for peaks in a wiggle file are calculated? I know the vertical axis in a wig file is its score but I'd like to know a bit about what it means. (I was asked about it by a colleague and I didn't have an answer).
Thanks!
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Leave a comment: