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Hi all,
I have some miRNA sequencing data, single end with 75bp read length. After I trimmed the 3' adapter, I found my read length distribution has two peaks, one is around 23 which I assume is correct, another peak is around 44 even higher than first one. Does it means my data quality is poor? Is there anything I could do for this?
I have some miRNA sequencing data, single end with 75bp read length. After I trimmed the 3' adapter, I found my read length distribution has two peaks, one is around 23 which I assume is correct, another peak is around 44 even higher than first one. Does it means my data quality is poor? Is there anything I could do for this?