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  • Can we sequence RNA directly

    Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now?

    Or now most sequencing center still use cDNA to do metatranscriptomics.

  • #2
    I know two technologies for direct transcriptome analysis:
    1- nanoString: it can only quantify known transcripts
    2- MinIon: the kit currently is offered for early access

    cDNA still is the most common method.

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    • #3
      Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. Direct RNA sequencing is a completely new way of analysing the sequence of RNA samples and it will improve the ease and speed of RNA analysis, while yielding richer biological information.

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      • #4
        Originally posted by nucacidhunter View Post
        1- nanoString: it can only quantify known transcripts
        nanoString doesn't "sequence" the RNA, unless you're referring to Hyb & Seq (which can sequence novel sequences, but is still in the technology development stages).

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