Originally posted by nucacidhunter
View Post
Unconfigured Ad
Collapse
X
-
nanoString doesn't "sequence" the RNA, unless you're referring to Hyb & Seq (which can sequence novel sequences, but is still in the technology development stages).
-
Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. Direct RNA sequencing is a completely new way of analysing the sequence of RNA samples and it will improve the ease and speed of RNA analysis, while yielding richer biological information.
Leave a comment:
-
I know two technologies for direct transcriptome analysis:
1- nanoString: it can only quantify known transcripts
2- MinIon: the kit currently is offered for early access
cDNA still is the most common method.
Leave a comment:
-
Can we sequence RNA directly
Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now?
Or now most sequencing center still use cDNA to do metatranscriptomics.Tags: None
Latest Articles
Collapse
-
by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
Channel: Articles
10-14-2026, 09:55 AM -
-
by SEQadmin2
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...-
Channel: Articles
-
ad_right_rmr
Collapse
News
Collapse
| Topics | Statistics | Last Post | ||
|---|---|---|---|---|
|
Started by SEQadmin2, 10-22-2026, 11:13 AM
|
0 responses
23 views
0 reactions
|
Last Post
by SEQadmin2
10-22-2026, 11:13 AM
|
||
|
Started by SEQadmin2, 10-22-2026, 10:48 AM
|
0 responses
20 views
0 reactions
|
Last Post
by SEQadmin2
10-22-2026, 10:48 AM
|
||
|
Started by SEQadmin2, 10-15-2026, 10:52 AM
|
0 responses
36 views
0 reactions
|
Last Post
by SEQadmin2
10-15-2026, 10:52 AM
|
||
|
Started by SEQadmin2, 10-14-2026, 05:39 AM
|
0 responses
42 views
0 reactions
|
Last Post
by SEQadmin2
10-14-2026, 05:39 AM
|
Leave a comment: