Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Calle
    Junior Member
    • Feb 2009
    • 5

    #1

    BWA --> SAM: sequence shown of strand where it maps shown

    Hi,

    I mapped some Illumina 75x2 reads using bwa (Version: 0.5.8). When I used samtools pileup I noticed that I have loads os variant calls everywhere. Upon further investigation I noticed that the nt-sequence in the sam file is presented on the strans where the read maps and not on the forward strand of the reference (as it should be?).

    I have not had this problem with pileups using any other aligner. Is this common behavior for bwa? If so, does anyone know what can be done?
    Am I missing something here?

    Thanks
    Last edited by Calle; 10-13-2010, 02:48 PM.

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    10-30-2026, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
45 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
75 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
49 views
0 reactions
Last Post SEQadmin2  
Working...