Hi all,
I use RNA2MAp pipeline to predict novels miRNA in horse genome using SOLID data. But, I dont understand how the filtering step work ? I know Mapread is use but how adapters are removed ? Only adapters in 3' are trimmed or all adapters ?
Moreover, the gff file is used for what ? What is the process ? all the reads are mapped in the reference genome and next knows miRNA
are identified and reads appears as unknows miRNA are save in a file ? Or only reads appears as knows miRNA are mapped in genome ?
I'd like use many reference files of miRBase with many organism, it's possible with RNA2Map ?
Thank you all,
Rachel
PS: sorry for my bad english !!
I use RNA2MAp pipeline to predict novels miRNA in horse genome using SOLID data. But, I dont understand how the filtering step work ? I know Mapread is use but how adapters are removed ? Only adapters in 3' are trimmed or all adapters ?
Moreover, the gff file is used for what ? What is the process ? all the reads are mapped in the reference genome and next knows miRNA
are identified and reads appears as unknows miRNA are save in a file ? Or only reads appears as knows miRNA are mapped in genome ?
I'd like use many reference files of miRBase with many organism, it's possible with RNA2Map ?
Thank you all,
Rachel
PS: sorry for my bad english !!
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