Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • leontovyc_roman
    Junior Member
    • Aug 2016
    • 5
    #1

    RSEM: differential expression of specific enzymes within transcriptome

    Hi
    I'm interested in specific proteolytic enzymes in transcriptomic data. I chose 3 different transcripts from de novo assembly and run RSEM analysis with pair-end data reads.
    First question: Is it Ok to run RSEM on such small set of reference sequences?

    Second question: How to deal with different length of reference sequences? In my case I had 980, 247 and 929 nucleotides. I run two analysis, one with original length of reference sequences, and second with trimmed sequences on 247 nucleotides for all sequences and I got different results.

    thanks in advance.

    Roman
    Tags: None
    Previous template Next

    Latest Articles

    Collapse
    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has      ...
      07-20-2026, 11:48 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Consortium Completes First Full Diploid Human Genome by SEQadmin2
    Started by SEQadmin2, 08-06-2026, 07:41 AM
    0 responses
    14 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    08-06-2026, 07:41 AM  
    Long-Read Sequencing Closes Decades-Old Gaps in the Medaka Genome by SEQadmin2
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    31 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    08-03-2026, 10:13 AM  
    New Genomic Method Uncovers Ancient Hominin DNA by SEQadmin2
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    40 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-31-2026, 02:55 AM  
    Study Captures the First Moments of DNA Replication by SEQadmin2
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    26 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-24-2026, 12:17 PM  
    View All
    Working...