Hi, all!
I have a dataset from Sanger (12 seqs ~350-500bp) and 454 sequencing (32000 seqs (140 OTUs) ~500bp).
And here is a question:
It's possible to compare OTUs seqs from 454 with Sanger by weighted\unweighted UniFrac analysis?
And one more question: It's correctly to do this if I have so different size of datasets - 12 seqs vs. 140 OTUs? Could I say that my 12 Sanger seqs are equivalent to OTUs in metagenome?
Thanks,
Kind regards!
I have a dataset from Sanger (12 seqs ~350-500bp) and 454 sequencing (32000 seqs (140 OTUs) ~500bp).
And here is a question:
It's possible to compare OTUs seqs from 454 with Sanger by weighted\unweighted UniFrac analysis?
And one more question: It's correctly to do this if I have so different size of datasets - 12 seqs vs. 140 OTUs? Could I say that my 12 Sanger seqs are equivalent to OTUs in metagenome?
Thanks,
Kind regards!
Comment