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  • sam/bam to bowtie

    I have files in bam format of reads mapped with BWA, I would like to know if there's any tool available to convert to bowtie format. I have a program for downstream analysis (for the collapse of clones) which is written for bowtie format, hence I need to convert the bam into bowtie. I would very much appreciate any guidance here.

    cheers,

    M

  • #2
    you will lose gapped alignment and mapping quality produced by bwa, which may be useful to downstream analyses.

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    • #3
      Hi,

      The program I want to use removes clones an re calculates the quality string, I guess I can go back to sam once I know what reads are not clonal so I dont loose all the information. Alternatively I could ask if there is a way to remove clones in sam/bam output.
      Thanks for your answer

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