Thank you dear all.
Indeed SRA prefers *.sff but meanwhile I found out, that they would also help you out, if you only can provide *.fna and *.qual.
I was also able to get all *.sffs now. The problem was a bug in the 2.0.00.20 software version of the 454 sequencer, which did not report the same IDs in the *.fna and the *.sff ...
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Originally posted by enna80 View PostThank you!
Jup, just as i thought.
Well, problem is that the NCBI's Sequence Read Archive (SRA) will only take *.sff but for one sample we do not have them.
Best
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The NCBI certainly won't want an SFF file with faked flow information.
I suggest you email the SRA to explain your situation, but first try to talk to your sequencing center again about getting the SFF files (and in future, insist on them).
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Thank you!
Jup, just as i thought.
Well, problem is that the NCBI's Sequence Read Archive (SRA) will only take *.sff but for one sample we do not have them.
Best
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The short answer is no, because you won't have the flow space information. You can fake this, but why bother? The Roche Newbler tools accept FASTA+QUAL as input don't they (as well as SFF)?
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creating Roche's sff files
Hello,
does anybody knows if *.sff files can be created for example from Roche's *.qual and *.fna files?
Have a nice day, enna80Tags: None
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