Hi,
I just wondered, are there any sequencing protocol kits around for shearing DNA by chemical means? I know that heating RNA in presence of divalent cations can be used to fragment RNA (i.e. Illumina TruSeq protocol kits). Do similar kits exist/or are methods applied for fragmenting DNA prior to sequencing?
Cheers,
Jamie
I just wondered, are there any sequencing protocol kits around for shearing DNA by chemical means? I know that heating RNA in presence of divalent cations can be used to fragment RNA (i.e. Illumina TruSeq protocol kits). Do similar kits exist/or are methods applied for fragmenting DNA prior to sequencing?
Cheers,
Jamie
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