Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • neavemj
    replied
    Hi lac302,

    Well, you could do something like this:

    Code:
    for j in *reads.fq; do deinterleave_fastq.sh < $j $(basename $j .fq).F.fq $(basename $j .fq).R.fq [compress]; done
    It's still a bit tricky to tell without seeing the full file name, or understanding completely how the program works, but I think this should do the job.

    Best,

    Matt.

    Leave a comment:


  • lac302
    replied
    Originally posted by neavemj View Post
    Maybe you could loop over each of the interleaved files and run the script? Something like:

    Code:
    for j in *fastq; do deinterleave_fastq.sh $j *options; done
    If you could tell me your directory structure and the command for how you normally run the program, I could be more specific.

    Cheers,

    Matt.
    Code:
    deinterleave_fastq.sh < interleaved.fastq f.fastq r.fastq [compress]
    The directory structure is just a folder of interleaved fastq files *0001reads.fq, *0002reads.fq, etc.

    Leave a comment:


  • neavemj
    replied
    Maybe you could loop over each of the interleaved files and run the script? Something like:

    Code:
    for j in *fastq; do deinterleave_fastq.sh $j *options; done
    If you could tell me your directory structure and the command for how you normally run the program, I could be more specific.

    Cheers,

    Matt.

    Leave a comment:


  • need to deinterleave/split all fastq files within a directory

    I've been using this script in the link below for several years when I need to split PE reads into separate R1 and R2 files.

    deinterleave FASTQ files. GitHub Gist: instantly share code, notes, and snippets.


    Does anyone know of a way to do the same thing recursively to a whole folder? I have 200 RNAseq samples that all need to be split.

Latest Articles

Collapse

  • seqadmin
    Addressing Off-Target Effects in CRISPR Technologies
    by seqadmin






    The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
    08-27-2024, 04:44 AM
  • seqadmin
    Selecting and Optimizing mRNA Library Preparations
    by seqadmin



    Sequencing mRNA provides a snapshot of cellular activity, allowing researchers to study the dynamics of cellular processes, compare gene expression across different tissue types, and gain insights into the mechanisms of complex diseases. “mRNA’s central role in the dogma of molecular biology makes it a logical and relevant focus for transcriptomic studies,” stated Sebastian Aguilar Pierlé, Ph.D., Application Development Lead at Inorevia. “One of the major hurdles for...
    08-07-2024, 12:11 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 08-27-2024, 04:40 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 08-22-2024, 05:00 AM
0 responses
293 views
0 likes
Last Post seqadmin  
Started by seqadmin, 08-21-2024, 10:49 AM
0 responses
135 views
0 likes
Last Post seqadmin  
Started by seqadmin, 08-19-2024, 05:12 AM
0 responses
124 views
0 likes
Last Post seqadmin  
Working...
X