Hi everyone,
Currently I started to study of DNA assembly and I found something I could not understand in the following paper:
The figure 1 in the paper shows the relationship between the quality score and the number of errors in reads. They said that the quality score 40 meant 0.01% of error probability and that was true it the following equation was used:
Q = -10log(p/(1-p))
However, according to the graph, only about 65% bases which have the score 40 are correct. Moreover, the percentage of correct bases which have smaller than 40 is almost 0 for all values. I wonder whether this trend is usual or not.
Thank you.
Currently I started to study of DNA assembly and I found something I could not understand in the following paper:
The figure 1 in the paper shows the relationship between the quality score and the number of errors in reads. They said that the quality score 40 meant 0.01% of error probability and that was true it the following equation was used:
Q = -10log(p/(1-p))
However, according to the graph, only about 65% bases which have the score 40 are correct. Moreover, the percentage of correct bases which have smaller than 40 is almost 0 for all values. I wonder whether this trend is usual or not.
Thank you.
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