Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
BBMAP paired-end alignment problem
Hi All
I'm having a problem with aligning Illumina PE reads with BBMap version 35.92.
Execution of
Code:bbmap.sh overwrite=t ref=Z.fasta in=R1_001_val_1.100.fq in2=R2_001_val_2.100.fq minid=0.25 mappedonly=true out=out.sam outu=unaligned_reads.fa maxindel=100000
Code:Pairing data: pct reads num reads pct bases num bases mated pairs: 100.0000% 100 107.4763% 59558 bad pairs: 0.0000% 0 0.0000% 0 insert size avg: 412.82 Read 1 data: pct reads num reads pct bases num bases mapped: 100.0000% 100 100.0000% 29779 unambiguous: 100.0000% 100 100.0000% 29779 ambiguous: 0.0000% 0 0.0000% 0 low-Q discards: 0.0000% 0 0.0000% 0 perfect best site: 0.0000% 0 0.0000% 0 semiperfect site: 35.0000% 35 35.1758% 10475 rescued: 0.0000% 0 Match Rate: NA NA 88.8176% 26449 Error Rate: 65.0000% 65 4.4763% 1333 Sub Rate: 65.0000% 65 0.3627% 108 Del Rate: 0.0000% 0 0.0000% 0 Ins Rate: 49.0000% 49 4.1136% 1225 N Rate: 100.0000% 100 6.7061% 1997 Read 2 data: pct reads num reads pct bases num bases mapped: 100.0000% 100 100.0000% 25636 unambiguous: 100.0000% 100 100.0000% 25636 ambiguous: 0.0000% 0 0.0000% 0 low-Q discards: 0.0000% 0 0.0000% 0 perfect best site: 0.0000% 0 0.0000% 0 semiperfect site: 26.0000% 26 25.3745% 6505 rescued: 2.0000% 2 Match Rate: NA NA 86.8232% 22258 Error Rate: 74.0000% 74 4.9852% 1278 Sub Rate: 74.0000% 74 0.8894% 228 Del Rate: 0.0000% 0 0.0000% 0 Ins Rate: 42.0000% 42 4.0958% 1050 N Rate: 100.0000% 100 8.1916% 2100
Code:bbmap.sh overwrite=t ref=Z.fasta in=R2_001_val_2.100.fq minid=0.25 mappedonly=true out=out.sam outu=unaligned_reads.fa maxindel=100000
Any ideas what is wrong with the first command line for the paired-end reads?
Thanks
Mark
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 07-25-2024, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
07-25-2024, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
28 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
161 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|
Leave a comment: