Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Unicycler

    I know Unicycler is specialised for bacterial genome assembling from hybrid of short and long reads.
    But, is Unicycler also good for bacterial genome assembling only from short reads such as from Illumina MiSeq

  • #2
    Yes, because it acts as an optimiser for Spades.. which is perfectly happy with just short read data.

    Comment


    • #3
      Thak you very much!
      I have one set paired (2 x250) Illumnia MiSeq data from Salmonella.
      They look good by FastaQC
      I did Unicycler assembling but I did not get a whole genome.
      The longest contig is 446213 and totally I got 59 contigs longer than 500 bp.
      What can I do next?

      Comment


      • #4
        Some minION sequencing?

        There's nothing wrong with that number of contigs from a 2x250 MiSeq run.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          The Impact of AI in Genomic Medicine
          by seqadmin



          Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
          02-26-2024, 02:07 PM
        • seqadmin
          Multiomics Techniques Advancing Disease Research
          by seqadmin


          New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

          A major leap in the field has
          ...
          02-08-2024, 06:33 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Today, 06:12 AM
        0 responses
        13 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 02-23-2024, 04:11 PM
        0 responses
        67 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 02-21-2024, 08:52 AM
        0 responses
        70 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 02-20-2024, 08:57 AM
        0 responses
        61 views
        0 likes
        Last Post seqadmin  
        Working...
        X