Hi guys,
I was wandering what is the right way to add the sequence of GFP reporter to a mouse genom. Basically instead of "geneA" in the genome the modified mouse has "geneA-IRES-GFP".
I guess that adding just the sequence of GFP as an extra chromosome is not ideal, since a read that would map to both "geneA" and "IRES-GFP" would not be mapped by STAR.
Does anyone know what to do?
Thanks
I was wandering what is the right way to add the sequence of GFP reporter to a mouse genom. Basically instead of "geneA" in the genome the modified mouse has "geneA-IRES-GFP".
I guess that adding just the sequence of GFP as an extra chromosome is not ideal, since a read that would map to both "geneA" and "IRES-GFP" would not be mapped by STAR.
Does anyone know what to do?
Thanks
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