Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • rahilsethi
    Member
    • May 2010
    • 22

    #1

    ViralFusionSeq not finding human-virus fusion regions

    I ran ViralFusionSeq, first on test dataset, given with the software package. I took one the contig assembly sequence generated by tool in the final contig output file. The contig sequence showed BLAST hits with human and hbv virus. I repeated the same procedure with HIV and human, on sample where I manually found clipped sequences that are shared between the two genomes. I then BLAST aligned contig sequences to both human and HIV virus. They only map to HIV. Why am I seeing this? The run was fine without any error reported. The data was DNASeq so I didn't provide any ORF, because I wanted to see all HIV-human integration regions, not only at specific ORF regions.

    viral.fusion.pl --config vfs.conf --thread 8 sample_name sample_trim_1.fq sample_trim2.fq

    Has anyone noticed such output?
    Attached Files

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Today, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
68 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...