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  • juan
    Member
    • Aug 2009
    • 14

    #1

    BWA 0.5.9rc1

    Being the early adopter, I tried using the -r option in bwa sampe from the latest version of bwa. If I understand correctly, this option will add the specified @RG tag to the SAM output file.
    Has anyone else tried this? How does it work? I tried adding the following option and get an error

    bwa sampe -P -a 1000 -r '@RG\tID:what\tCN:where\tDS:why\tDT:when\tPL:ILLUMINA\tPU:how\tSM:who' -f f1.sam human_g1k_v37.fasta f1_1.sai f1_2.sai f1.fq f2.fq

    The error I get is
    [bam_header_read] invalid BAM binary header (this is not a BAM file).
  • Mali Salmon
    Member
    • Jul 2008
    • 24

    #2
    Got the same error (using the same version), even without using -r

    Comment

    • jyli
      Member
      • Nov 2008
      • 21

      #3
      Originally posted by juan View Post
      Being the early adopter, I tried using the -r option in bwa sampe from the latest version of bwa. If I understand correctly, this option will add the specified @RG tag to the SAM output file.
      Has anyone else tried this? How does it work? I tried adding the following option and get an error

      bwa sampe -P -a 1000 -r '@RG\tID:what\tCN:where\tDS:why\tDT:when\tPL:ILLUMINA\tPU:how\tSM:who' -f f1.sam human_g1k_v37.fasta f1_1.sai f1_2.sai f1.fq f2.fq

      The error I get is
      [bam_header_read] invalid BAM binary header (this is not a BAM file).
      Hi, there

      I tried similar thing, but it gave me different errors:

      bwa sampe -P -a 334 -r '@RG\tID:dodrp445981_1_2_13052011\tSM:dodrp445981\tLB:ga\tPL:Illumina'

      Error:

      malformated @RG line

      Can anyone help here??

      Comment

      • jyli
        Member
        • Nov 2008
        • 21

        #4
        Originally posted by juan View Post
        Being the early adopter, I tried using the -r option in bwa sampe from the latest version of bwa. If I understand correctly, this option will add the specified @RG tag to the SAM output file.
        Has anyone else tried this? How does it work? I tried adding the following option and get an error

        bwa sampe -P -a 1000 -r '@RG\tID:what\tCN:where\tDS:why\tDT:when\tPL:ILLUMINA\tPU:how\tSM:who' -f f1.sam human_g1k_v37.fasta f1_1.sai f1_2.sai f1.fq f2.fq

        The error I get is
        [bam_header_read] invalid BAM binary header (this is not a BAM file).
        Originally posted by jyli View Post
        Hi, there

        I tried similar thing, but it gave me different errors:

        bwa sampe -P -a 334 -r '@RG\tID:dodrp445981_1_2_13052011\tSM:dodrp445981\tLB:ga\tPL:Illumina'

        Error:

        malformated @RG line

        Can anyone help here??
        Actually, I found the piece of C code in bwa, BUT, I never programmed in "C"

        while ((c = getopt(argc, argv, "a:sPn:N:c:f:Ar:")) >= 0) {
        switch (c) {
        case 'r':
        if (bwa_set_rg(optarg) < 0) {
        fprintf(stderr, "[%s] malformated @RG line\n", __func__);
        return 1;
        }
        break;

        Comment

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