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ksw9

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Join Date: May 2013

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Best programs for Sanger sequence alignment, annotation?

11-21-2018, 09:12 AM

Dear all,
I'm wondering what your recommendations are for best programs to use for multiple sequence alignment and annotation of Sanger sequences? My experience is with NGS data, so I have not yet used many of the tools for Sanger sequences ~1000bp long.

Thank you in advance!
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Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by GATTACAT

Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Nine Things a Sample Prep Scientist Thinks About Before Sequencing

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