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  • adaptivegenome
    Super Moderator
    • Nov 2009
    • 436

    #1

    Best way to combine sequence data from two genomes?

    Guys,

    Sorry for the possibly naive question, but I have two genomes sequenced by Illumina. Each is supposedly homozygous due to inbreeding. I want to combine the two genomes together to simulate heterozygosity.

    I made BAM files of each genome using BWA.

    I suppose I could use "samtools merge" to combine the two BAM files but is this really fair? Is there a better way to do this, perhaps at the level of BWA?

    Thanks!

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