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I did some searching and came up with very little about how to effectively demultiplex illumina data off the GAiix platform. Does anyone know of a good software package or workflow I could read through and experiment with? Sorry if this question has already been asked, I wasn't able to find much information though
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Are you using your own barcodes or the Illumina multiplexing kit? The most recent version of NextGENe handles the demultiplexing of the data from the kits. I'm also trying to get it to work with CLC Genomics Workbench, but so far they aren't sorting right. Both of these programs cost $$ and I'm unfortunately not aware of free software that does it, although I'm sure it exists and someone else might know. Illumina software (CASAVA) will demultiplex data generated from their kits. It sorts into bins 1-24 (or 1-48?) but make sure to check what adapter/barcode is sorted into each bin because the numbers 1-24 don't correspond to adapters 1-24. I hope that helps!
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Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM
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