Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SOAPdeNovo, get the contigs

    Hello all,
    I am writing this message because I will need help with SOAPDeNovo. Indeed, I made an assembly of Novo with this tool and I want to recover the final contigs. I'm outputting two files. The first file in .contig corresponding to contig sequences without using mate pair information . The second file is in .ScafSeq containing scaffold sequences.
    On the SOAP website , it is specified that the final contigs can be extracted by decomposing the scaffold sequences at the level of the gap regions.
    I would like to know how to achieve this decomposition in order to get the contigs?
    Hoping that you could help me I wish you a great day.
    Cordially
    (Sorry for my bad level in english)

  • #2
    Why not use the scaffolds ? These are the final contigs organised into scaffolds by using the supplied probably mate pair info. This is what I have always used.

    If you still want the contigs, maybe just break the scaffolds at long N stretches ?

    If its an illumina assembly, it is likely to be pretty awful. Long reads are the way to go these days as I'm sure you know.

    Comment


    • #3
      Hello, I want to thank you for your answer.
      I wanted to recover contigs because I want to scaffolding from two files containing separate contigs.
      Thank you very much for your response and your help

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:57 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-06-2024, 07:17 AM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-02-2024, 08:06 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-30-2024, 12:17 PM
      0 responses
      24 views
      0 likes
      Last Post seqadmin  
      Working...
      X