Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Loop in bwa commands

    Hiya,

    I'm trying to loop multiple input files with matching prefixes and different file types in bwa sampe ; here's the general structure:
    Code:
    bwa sampe /Users/xxx/Desktop/Index_align/GRCh37_latest_genomic.fna H2_S16_L001_read1.sai H2_S16_L001_read2.sai \
    H2_S16_L001_R1_001.fastq.gz H2_S16_L001_R2_001.fastq.gz > aln_H2_S16_L001.sam
    I'm trying to make a loop like:
    Code:
    for i in /Users/xxx/Desktop/Index_align/Fastq/fastq_run4/ \
    do bwa sampe /Users/xxx/Desktop/Index_align/GRCh37_latest_genomic.fna \
    $i\-read1.sai $i\-read2.sai $i\-R1_001.fastq.gz $i\-R2_001.fastq.gz > $i\-aln.sam; done
    Does anyone have suggestions for what I am missing? Like perhaps I need to create a list of the prefix file names? I would greatly appreciate any advice. Thanks!

  • #2
    You can do the following:
    for f in /Users/xxx/Desktop/Index_align/Fastq/fastq_run4/*.fastq.gz; do
    name=$(basename $f _L001_R1_001.fastq.gz)
    bwa sampe /Users/xxx/Desktop/Index_align/GRCh37_latest_genomic.fna \
    ${name}_L001_read1.sai ${name}_L001_read2.sai \
    ${name}_L001_R1_001.fastq.gz ${name}_L001_R2_001.fastq.gz > ${name}_aln.sam
    done

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Advanced Methods for the Detection of Infectious Disease
      by seqadmin




      The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
      ...
      11-27-2023, 01:15 PM
    • seqadmin
      Strategies for Investigating the Microbiome
      by seqadmin




      Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
      11-09-2023, 07:02 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 09:55 AM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 11-30-2023, 10:48 AM
    0 responses
    17 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 11-29-2023, 08:26 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 11-29-2023, 08:12 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Working...
    X