Hi All
One of our local lab groups has taken the jump to start doing scRNA-seq and has asked for assistence in analysing their data.
Becuase it is 10x data we settled on using cellranger followed by Seurat as the initial starting point to do some clustering and expression testing.
I'm more than happy with how to get data into Seurat and do clustering in general but am looking for advice on what would be the best way to input and process data from 4 patients and 4 controls with the aim of being able to test between the two groups.
One of our local lab groups has taken the jump to start doing scRNA-seq and has asked for assistence in analysing their data.
Becuase it is 10x data we settled on using cellranger followed by Seurat as the initial starting point to do some clustering and expression testing.
I'm more than happy with how to get data into Seurat and do clustering in general but am looking for advice on what would be the best way to input and process data from 4 patients and 4 controls with the aim of being able to test between the two groups.