Seqanswers Leaderboard Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Danil S.
    Junior Member
    • Mar 2020
    • 1

    Bac ends profiling to a genome

    Hi,

    I am looking for the pipeline which provides bac-ends profiling to the reference genome.

    I used to work with illumina myseq 16s rRNA paired-end sequences.And i used qiime2 to treat it. Now i have a sanger bac-end library of the whole genome and i need to map them to a genome sequence in order to be able to choose BAC with gene of interest.

    I suppose that what i have to do is:

    1.Filter out sequences that are 95% similarity or more to each other.

    2.Remoove sequences which contain repeats.

    3.Map remaining paired sequences.

    4.Leave paires of bac-ends with unique hits only.

    Thus i can choose a BAC which contains gene of interest for the further research. In theory.

    Can anyone suggest tools for this work and maybe correct my "pipeline"?


    Thank you!
    Last edited by Danil S.; 03-01-2020, 04:12 AM.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Cross-posted and answered on Biostars: https://www.biostars.org/p/424091/

    Comment

    Latest Articles

    Collapse

    • seqadmin
      New Genomics Tools and Methods Shared at AGBT 2025
      by seqadmin


      This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

      The Headliner
      The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
      03-03-2025, 01:39 PM
    • seqadmin
      Investigating the Gut Microbiome Through Diet and Spatial Biology
      by seqadmin




      The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
      02-24-2025, 06:31 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-20-2025, 05:03 AM
    0 responses
    17 views
    0 reactions
    Last Post seqadmin  
    Started by seqadmin, 03-19-2025, 07:27 AM
    0 responses
    18 views
    0 reactions
    Last Post seqadmin  
    Started by seqadmin, 03-18-2025, 12:50 PM
    0 responses
    19 views
    0 reactions
    Last Post seqadmin  
    Started by seqadmin, 03-03-2025, 01:15 PM
    0 responses
    185 views
    0 reactions
    Last Post seqadmin  
    Working...