Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SRMA memory leak

    Hi friends
    Was anybody successful using SRMA? I have tried it with the RANGE option sa well, and it has worked for chr1, but I get the error with chr2.. Here is the error (RAM we have is 140GB) after that srcript is hanging:


    [Wed Mar 02 09:28:35 CET 2011] srma.SRMA INPUT=[camergdup.bam] OUTPUT=[casrmachr2.bam] REFERENCE=/project/erasys1/ngs/ca/hg19.fa RANGE=chr2:1-243199373 OFFSET=20 MIN_MAPQ=0 MINIMUM_ALLELE_PROBABILITY=0.1 MINIMUM_ALLELE_COVERAGE=3 MAXIMUM_TOTAL_COVERAGE=100 CORRECT_BASES=false USE_SEQUENCE_QUALITIES=true QUIET_STDERR=false MAX_HEAP_SIZE=8192 MAX_QUEUE_SIZE=65536 NUM_THREADS=1 TMP_DIR=/tmp/ot VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false
    Allele coverage cutoffs:
    coverage: 1 minimum allele coverage: 0
    coverage: 2 minimum allele coverage: 0
    coverage: 3 minimum allele coverage: 0
    coverage: 4 minimum allele coverage: 1
    coverage: 5 minimum allele coverage: 1
    coverage: 6 minimum allele coverage: 1
    coverage: 7 minimum allele coverage: 2
    coverage: 8 minimum allele coverage: 2
    coverage: 9 minimum allele coverage: 3
    coverage: >9 minimum allele coverage: 3
    Records processsed: 2752469 (last chr2:231988063-231988112)Exception in thread "Thread-84" java.lang.OutOfMemoryError: Java heap space
    at java.util.PriorityQueue.<init>(Unknown Source)
    at srma.Graph.addNode(Graph.java:161)
    at srma.Graph.addSAMRecord(Graph.java:120)

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:35 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-09-2024, 02:46 PM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
18 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-06-2024, 07:17 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Working...
X