Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • dnusol
    Senior Member
    • Jul 2009
    • 136

    #1

    possible error in cufflinks?

    Hi, after running cufflinks and cuffcompare on my arabidopsis experiment, I think I found some inconsistencies between the combined.gtf file and the sample.tmap generated. The Cufflinks gene id does not match across the two files. See this example:

    Code:
    grep -w 'MEE4' transcripts.tmap 
    MEE4    AT1G04630.1     c       CUFF.1011       CUFF.1011.1     100     194.737485      166.827810      222.647159      91.465400       841     CUFF.1011.1
    
    grep -w 'MEE4' cuffcom-out-all-sinG.combined.gtf 
    Chr1    Cufflinks       exon    1289694 1290223 .       -       .       gene_id "XLOC_026901"; transcript_id "TCONS_00084569"; exon_number "1"; gene_name "MEE4"; oId "CUFF.993.1"; nearest_ref "AT1G04630.1"; class_code "c"; tss_id "TSS36322";
    Chr1    Cufflinks       exon    1290772 1291077 .       -       .       gene_id "XLOC_026901"; transcript_id "TCONS_00084569"; exon_number "2"; gene_name "MEE4"; oId "CUFF.993.1"; nearest_ref "AT1G04630.1"; class_code "c"; tss_id "TSS36322";
    Chr1    Cufflinks       exon    1289715 1290065 .       -       .       gene_id "XLOC_026901"; transcript_id "TCONS_00094944"; exon_number "1"; gene_name "MEE4"; oId "CUFF.1007.1"; nearest_ref "AT1G04630.1"; class_code "="; tss_id "TSS36322";
    Chr1    Cufflinks       exon    1290152 1290223 .       -       .       gene_id "XLOC_026901"; transcript_id "TCONS_00094944"; exon_number "2"; gene_name "MEE4"; oId "CUFF.1007.1"; nearest_ref "AT1G04630.1"; class_code "="; tss_id "TSS36322";
    Chr1    Cufflinks       exon    1290772 1291089 .       -       .       gene_id "XLOC_026901"; transcript_id "TCONS_00094944"; exon_number "3"; gene_name "MEE4"; oId "CUFF.1007.1"; nearest_ref "AT1G04630.1"; class_code "="; tss_id "TSS36322";
    Has anyone seen this before? I have not checked all the way through the files but it also happens in another gene I looke for

    I am using cufflinks 0.9.2

    Thanks,

    Dave

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM
  • SEQadmin2
    Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
    by SEQadmin2



    Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
    ...
    07-09-2026, 11:10 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Today, 10:13 AM
0 responses
10 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-31-2026, 02:55 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-24-2026, 12:17 PM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-23-2026, 11:41 AM
0 responses
17 views
0 reactions
Last Post SEQadmin2  
Working...