Seqanswers Leaderboard Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • PFS
    Member
    • Mar 2010
    • 55

    coverage depth, bedtools and RNA-Seq experiment

    I want to estimate the % of a transcriptome covered by an RNA-Seq experiment.

    I tried to use BEDTools: one file consisted of exons only coordinates (from UCSC), the other file consisted of TopHat alignments.

    The results show that 70% of the transcriptome has coverage depth 0.

    I was expecting a much smaller percentage, given that I started with 100,000,000 reads x 2 (PE) and the total length of the transcriptome is 17,470,227.

    Am I making a big mistake here?

    ps. when I say "transcriptome", I mean the collection of all exons.
  • natstreet
    Member
    • Nov 2009
    • 83

    #2
    Can you show us the command you used to generate that value?

    Comment

    • PFS
      Member
      • Mar 2010
      • 55

      #3
      Originally posted by natstreet View Post
      Can you show us the command you used to generate that value?
      coverageBed -abam my_accepted_hits.bam -b rn4_UCSC_kg_exons.bed -hist -s -split

      Comment

      Latest Articles

      Collapse

      • seqadmin
        New Genomics Tools and Methods Shared at AGBT 2025
        by seqadmin


        This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

        The Headliner
        The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
        03-03-2025, 01:39 PM
      • seqadmin
        Investigating the Gut Microbiome Through Diet and Spatial Biology
        by seqadmin




        The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
        02-24-2025, 06:31 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-20-2025, 05:03 AM
      0 responses
      20 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-19-2025, 07:27 AM
      0 responses
      26 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-18-2025, 12:50 PM
      0 responses
      19 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-03-2025, 01:15 PM
      0 responses
      187 views
      0 reactions
      Last Post seqadmin  
      Working...