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  • nsalomonis
    Junior Member
    • Feb 2011
    • 4

    #1

    De novo exon quantification from BAM files

    I am wondering if there is software that can predict which exons are present (clustering of reads) from TopHat BAM files and return associated counts for those predicted exons. I know BEDTools will do this for supplied known exons and that Cufflinks predicts novel exons, but I'm not sure how to get novel exon predictions and associated counts. Without these, I wouldn't want to trust novel junction predictions from programs like TopHat (need confirmatory data that a novel exon is present and expressed).

    Thanks in advance!

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