There are already some in depth discussions of storage and compute architectures on this forum, I'd have a look through those to see what others have opted for.
We're installing storage with a wedge of 15k drives for analysis with hopefully sufficient IO and a larger group of cheaper 2TB SATA drives for archiving. Data from the sequencer should in my opinion be directly to the machine provided by the supplier, and cleaned up after every run.
Keep in mind a dedicated network is important.
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Storage Requirements
Our company is looking at purchasing an Illumina NGS. They have told our IT department they only need us to provide low cost storage to store the result data.
I know the sequencer comes with a workstation with about 4TB of data, but what I'm trying to figure out what type of storage they will need to store the data and perform analysis. Are there specific performance requirements (IOPS, Aggregate throughput) in order to run analysis of the data?
Is it better to have the instrument write the data directly to the same storage that you would run the analysis from or to seperate it out?
We already have an Isilon cluster however they say the cost is too high for storage so I was looking at alternative solutions but want to make sure we purchase something that will not cause performance issues.
Any feedback from others is greatly appreciated.
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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