I have some 454 Titanium reads (not a paired lib, unfortunately) that I am aligning to the reference bacterial genome. I'd like to identify translocations, inversions and large indels, if possible using these data, but most methods that I've seen use paired reads for this. It seems to me that long reads (Sanger, titanium) could be useful for this too, if the read alignment is split, and each split aligns to two different locations in the reference genome. Does anyone know of a software that uses this approach or a paper that describes it?
Mike
Mike
Comment