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  • One problem about RefSeq annotation file

    Hi Guys,

    I have one question about the RefSeq annotation file downed from UCSC.

    Take the mm9 as example,

    Firstly, I downloaded the RefSeq GTF file of mm9 genome from the UCSC website and named it mm9_RefSeq.

    Then I wanted to see if the file format is standard, so I uploaded the file, namely mm9_RefSeq to the UCSC again. But an error jumped out with

    'Error File 'mm9_RefSeq' - GFF/GTF group NM_001031622_dup2 on chr5_random-, this line is on chrUn_random+, all group members must be on same seq and strand'

    As I didn't know much about either chrUn_ramdom or chrN_ramdom, I searched them and found them on the UCSC FAQ board,


    So my question is should I delete the entries referring to chrUn_ramdom and chrN_ramdom from the standard RefSeq annotation file before uploading it to the UCSC genome browser?

    And I also wanted to know what information could be observed from the chrUn_ramdom and chrN_ramdom entries in mm9_RefSeq. Could anybody please give me some ideas about it?

    Thanks a lot,

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