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Date and time: Thursday, September 20, 2012 8:00
Pacific Daylight Time
Program: Genomics eSeminar Series
Panelist(s) Info: Maria Celeste M. Ramirez, Ph.D., Genomics Application Scientist
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Formalin-fixed paraffin embedded (FFPE) samples represent a potential gold mine of information. Interrogation of these samples, which are usually tissue from various diseased states, through retrospective studies, can result in valuable information that can enable elucidation of molecular events that are linked to disease pathogenesis or clinical outcome as a result of a specific course of treatment. With the advent of next-generation sequencing (NGS), a highly sensitive and accurate technology that results in single-molecule resolution of variants, analysis of FFPE-derived samples offers even more promise as variations in highly heterogenous samples such as tumors can now be dissected on a molecular level. Despite this, there have been a lot of questions that surround the applicability of these samples for NGS-based analysis. For target enrichment especially, wherein the signal within the regions of interest are amplified, these questions become an even bigger concern. In this talk, we will cover the various considerations one has to address in order to take these FFPE-derived samples into two popular target enrichment workflows, SureSelect and Haloplex. Once addressed, these samples can then be taken into these workflows with confidence, resulting in valuable information that can be used to further our understanding of these various disease mechanisms and their impact on treatment.
Pacific Daylight Time
Program: Genomics eSeminar Series
Panelist(s) Info: Maria Celeste M. Ramirez, Ph.D., Genomics Application Scientist
LEARN MORE AND REGISTER NOW
Formalin-fixed paraffin embedded (FFPE) samples represent a potential gold mine of information. Interrogation of these samples, which are usually tissue from various diseased states, through retrospective studies, can result in valuable information that can enable elucidation of molecular events that are linked to disease pathogenesis or clinical outcome as a result of a specific course of treatment. With the advent of next-generation sequencing (NGS), a highly sensitive and accurate technology that results in single-molecule resolution of variants, analysis of FFPE-derived samples offers even more promise as variations in highly heterogenous samples such as tumors can now be dissected on a molecular level. Despite this, there have been a lot of questions that surround the applicability of these samples for NGS-based analysis. For target enrichment especially, wherein the signal within the regions of interest are amplified, these questions become an even bigger concern. In this talk, we will cover the various considerations one has to address in order to take these FFPE-derived samples into two popular target enrichment workflows, SureSelect and Haloplex. Once addressed, these samples can then be taken into these workflows with confidence, resulting in valuable information that can be used to further our understanding of these various disease mechanisms and their impact on treatment.