In a few months I will be setting up my 3rd NGS sequencing facility -- this time starting from scratch. I have a HiSeq system on the way, but need to select a technology for performing confirmation sequencing.
Do I drop the money to purchase an AB capillary sequencer (ie. 3500xL or 3730) --- or could I use another small scale NGS platform for confirmation ?
We'll be performing exome and genome sequencing using the HiSeq system -- could the identified variants be confirmed using a MiSeq ? Or would it be preferable to using a different technology, such as the Ion Torrent PGM ?
Or would it still be best to use traditional, capillary-based Sanger sequencing ?
I know Sanger sequencing will be around for quite a while, but I'm wondering if I could do confirmation sequencing more cheaply on a small-scale NGS system.
I welcome your opinions !
Thank you,
Mike
Do I drop the money to purchase an AB capillary sequencer (ie. 3500xL or 3730) --- or could I use another small scale NGS platform for confirmation ?
We'll be performing exome and genome sequencing using the HiSeq system -- could the identified variants be confirmed using a MiSeq ? Or would it be preferable to using a different technology, such as the Ion Torrent PGM ?
Or would it still be best to use traditional, capillary-based Sanger sequencing ?
I know Sanger sequencing will be around for quite a while, but I'm wondering if I could do confirmation sequencing more cheaply on a small-scale NGS system.
I welcome your opinions !
Thank you,
Mike
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