Hello all,
I need your suggestion about this result I got from a de novo transcriptome analysis using Newbler to gain best results (I think by improving N5).
I changed min. overlap length each time as in the table below and have the following quesitons:
What do you think, will this have an impact on the analysis?.
Which criterion we may use to gain best result for further analysis (i.e. # of contigs, N50....ect).
cheers,
I need your suggestion about this result I got from a de novo transcriptome analysis using Newbler to gain best results (I think by improving N5).
I changed min. overlap length each time as in the table below and have the following quesitons:
What do you think, will this have an impact on the analysis?.
Which criterion we may use to gain best result for further analysis (i.e. # of contigs, N50....ect).
HTML Code:
Mi.Overlap Length #duplicate N50 Total contigs mean Total seq. 35 106 912 15056 604.8 9106339 40 136 904 15417 592.5 9134740 45 132 899 15487 590.8 9152710 50 113 893 15537 589.3 9155956
Comment