I hear "multiplex" often, as in multitemplate PCR, multiplex PCR. What exactly is a plex?
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It isn't really a biological term, it's an english language thing. It shows that something has more than one part. So something that is duplex has two parts, while something that is multiplex has many parts. Multiplex PCR is several pairs of primers in one tube to amplify several different products from the same starting DNA.
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Originally posted by henry.wood View PostIt isn't really a biological term, it's an english language thing. It shows that something has more than one part. So something that is duplex has two parts, while something that is multiplex has many parts. Multiplex PCR is several pairs of primers in one tube to amplify several different products from the same starting DNA.
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Multiplexing means that you sequence several samples in one flow-cell lane. To this end, you label each sample with a "barcode" (or "multiplex tag") by ligating adapters which contain the barcode in addition to the Illumina sequencing primers, so that the barcode will end up getting sequenced as if it were part of your fragment. Later, in analysis, you trim of the barcode and sort your reads according to it.
With HiSeq machine producing more tens of millions of reads, using a full lane for only one sample is often overkill, and multiplexing saves cost.
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Originally posted by Simon Anders View PostMultiplexing means that you sequence several samples in one flow-cell lane. To this end, you label each sample with a "barcode" (or "multiplex tag") by ligating adapters which contain the barcode in addition to the Illumina sequencing primers, so that the barcode will end up getting sequenced as if it were part of your fragment. Later, in analysis, you trim of the barcode and sort your reads according to it.
With HiSeq machine producing more tens of millions of reads, using a full lane for only one sample is often overkill, and multiplexing saves cost.
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Originally posted by mandova View PostTHx!! So what is the consequence of using multiplex PCR instead of multitemplate PCR (if there's a technology that does) when a sequencing library is amplified...meaning, the implication of "multiplexing" in sequencing? Increased parallelism?
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