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  • nilshomer
    replied
    Originally posted by fadista View Post
    Hi,

    I would like to know of a good and fast way that could help me calculate the alignable portion of a genome (not human), given a reference sequence.
    When I say alignable portion I mean that I want to know all the positions of the genome that can be covered uniquely by reads of 36 bp and up to 2 mismatches.

    Best regards,
    João
    There are tools in BFAST (https://secure.genome.ucla.edu/index.php/BFAST) in the "butil" directory to solve exactly the problem described above. They include "bindexhist" and "bindexdist", which are documented in the accompanying reference manual.

    Leave a comment:


  • fadista
    started a topic alignable portion of a genome

    alignable portion of a genome

    Hi,

    I would like to know of a good and fast way that could help me calculate the alignable portion of a genome (not human), given a reference sequence.
    When I say alignable portion I mean that I want to know all the positions of the genome that can be covered uniquely by reads of 36 bp and up to 2 mismatches.

    Best regards,
    João

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